bcl2 rabbit ab  (Bioss)

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The mRNA expression of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2 , and Caspase 3 in E.tenella host cells.

Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

Techniques: Expressing

Journal: Poultry Science

Article Title: Pathogenic mechanism of Eimeria tenella Et MIC2 promotes Eimeria tenella invasion and inhibits host cell apoptosis through binding to the ITGAV receptor

doi: 10.1016/j.psj.2026.106922

Figure Lengend Snippet: The protein activity changes of ITGAV, FAK, PLC, PKC, p65, ERK, JNK, p38, PI3K, Akt, Bax, Bcl2, and Caspase 3 in E.tenella host cells.

Article Snippet: Bcl2 Rabbit Ab , Bioss , bs-0032R , 1: 1500.

Techniques: Activity Assay

Journal: Renal Failure

Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

doi: 10.1080/0886022X.2026.2652103

Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB after renal I/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus DS group; # p < 0.05 versus DI/R group; ■ p < 0.05 versus DI/R + RSV group.

Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

Techniques: Expressing, Western Blot

Journal: Renal Failure

Article Title: Resveratrol inhibits renal ischemia and reperfusion injury in diabetes via reducing oxidative stress, inflammation, and apoptosis

doi: 10.1080/0886022X.2026.2652103

Figure Lengend Snippet: The effects of RSV on the expression of Bcl-2, cleaved caspase-3, Bax, Nrf2, HO-1, TLR4, and NF-κB in HK-2 cells after H/R. (A) Representative Western blotting and (B–H) Quantification of Western blotting data. Data are expressed as mean ± SD. * p < 0.05 versus Control group; # p < 0.05 versus H/R group; ■ p < 0.05 versus H/R + RSV group.

Article Snippet: The blots were blocked with 5% skimmed milk and incubated with primary antibodies against Nrf2 (cat. no. 16396-1-AP; 1:2,000), NF-κB p65 (cat. no. 80979-1-RR; 1:1,000), HO-1 (cat. no. ab68477; 1:1,000), lamin B (cat. no. 66095-1-Ig; 1:20,000), β-actin (cat. no. 60004-1-Ig; 1:5,000; all Proteintech Group, Inc.), TLR4 (cat. no. AF7017; 1:1,000; Affinity Biosciences), B-cell lymphoma-2 (Bcl-2; cat. no. GB154830 ; 1:1,000; Servicebio), Bcl-2-associated X protein (Bax; cat. no. BS-0127R; 1:1,000; BIOSS), and cleaved caspase-3 (cat. no. 9661 T; 1:1,000; Cell Signaling Technology), respectively.

Techniques: Expressing, Western Blot, Control

Journal: Food Science & Nutrition

Article Title: Protective Effect of Silymarin Against Paclitaxel‐Induced Cardiotoxicity

doi: 10.1002/fsn3.71710

Figure Lengend Snippet: Relative protein expression of P2X7R, NfkB‐p65, Bcl‐2, TNF‐α, IL‐1β, and Caspase‐3 in rat heart tissues. Different superscripts (a–c) in the same row indicate a significant difference ( p < 0.05) between the groups.

Article Snippet: The membranes were blocked with 5% w/v bovine serum albumin for 90 min. Primary antibodies (IL‐1β [sc‐52,012], TNF‐α [sc‐52,746], Caspase‐3 [sc‐56,053], Bcl‐2 [sc‐7382], NF‐κB‐p65 [sc‐109], and beta‐actin [sc‐47,778], Santa Cruz Biotechnology, USA; P2X7R [11144–1‐AP], Proteintech Group, USA) were applied to the membranes and incubated at 4°C for approximately 15 h. Following a wash with Tris‐buffered saline containing Tween, the membranes were exposed to secondary antibodies (sc‐2004/sc‐2005, Santa Cruz Biotechnology, USA) conjugated with horseradish peroxidase (TP‐125‐HL, Thermo Fisher Scientific, USA) for 90 min. β‐actin (1:2000 dilution) was used as the loading control.

Techniques: Expressing

Journal: Experimental and Therapeutic Medicine

Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death

doi: 10.3892/etm.2026.13080

Figure Lengend Snippet: Myrrh reduces the growth of human endometriotic cells by activating apoptotic signaling pathways. Endometriotic cells (12Z) and normal endometrial cells (T-HESC) were treated with the indicated concentrations of myrrh. (A) Cell viability was analyzed by measuring at 450 nm wavelength using MTT after 24 h. (B) To assess apoptotic or dead cell populations, 12Z cells were treated with increasing doses of myrrh for 24 h, and the percentages of Annexin V-positive and PI-positive cells were measured (Annexin V: Ex 494/Em 525 nm; PI: Ex 535/Em 617 nm). After 24 h of treatment with each concentration of myrrh, mitochondria-associated apoptotic proteins were analyzed by immunoblotting. (C) Representatives immunoblot images of Bax and Bcl-2 are shown. (D) A major monomeric Bax band at ~21 kDa (monomer Bax) and higher-molecular-weight bands corresponding to oligomeric forms (tetramer Bax, ~84 kDa) are indicated. (E) Representative immunoblot images of caspase-3, caspase-9 and PARP are shown. GAPDH was used as an internal control. (F) Densitometric analysis of protein levels. Data are expressed as relative intensity compared to control. Data are expressed as mean ± standard error of the mean. Statistical analysis was performed using one-way ANOVA followed by Tukey's post hoc test. * P<0.05, ** P<0.01 and *** P<0.001. Ex, excitation; Em, emission; ; PARP, poly(ADP-ribose) polymerase.

Article Snippet: Subsequently. the membranes were subjected to overnight incubated at 4 ̊C with primary antibodies, including anti-human poly (ADP-ribose) polymerase (PARP, #9542s; Cell Signaling Technology, Inc.), caspases-3 (#9665s; Cell Signaling Technology, Inc.), caspase-9 (#9508s; Cell Signaling Technology, Inc.), Bax (NB100-56095; Novus Biologicals), Bcl-2 (NB100-56098; Novus Biologicals), p53 (sc-6243; Santa Cruz Biotechnology, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, sc-32233; Santa Cruz Biotechnology, Inc.).

Techniques: Protein-Protein interactions, Concentration Assay, Western Blot, Molecular Weight, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Myrrh ameliorates endometriosis by enhancing ER stress-related apoptotic cell death

doi: 10.3892/etm.2026.13080

Figure Lengend Snippet: Mechanism of action of myrrh in endometriosis. (A) 12Z were treated with the indicated concentrations of myrrh and TUDCA (200 µM). Cell viability was analyzed using MTT after 24 h, measured at a wavelength of 450 nm. (B) Schematic representation summarizing the experimental results and the potential mechanism by which myrrh exerts palliative effects on endometriosis. *** P<0.001. TUDCA, tauroursodeoxycholic acid; ATF6, activating transcription factor 6; IRE1α, inositol-requiring enzyme 1 alpha; ATF4, activating transcription factor 4; CHOP, C/EBP homologous protein; GADD34, growth arrest and DNA damage-inducible protein 34; Bax, Bcl-2-associated X protein; Bcl2, B-cell lymphoma 2; Caspase 3, cysteine-aspartic acid protease 3; Caspase 9, cysteine-aspartic acid protease 9; PARP, poly(ADP-ribose) polymerase.

Article Snippet: Subsequently. the membranes were subjected to overnight incubated at 4 ̊C with primary antibodies, including anti-human poly (ADP-ribose) polymerase (PARP, #9542s; Cell Signaling Technology, Inc.), caspases-3 (#9665s; Cell Signaling Technology, Inc.), caspase-9 (#9508s; Cell Signaling Technology, Inc.), Bax (NB100-56095; Novus Biologicals), Bcl-2 (NB100-56098; Novus Biologicals), p53 (sc-6243; Santa Cruz Biotechnology, Inc.) and glyceraldehyde 3-phosphate dehydrogenase (GAPDH, sc-32233; Santa Cruz Biotechnology, Inc.).

Techniques: